Regioselective deglycosylation of onion quercetin glucosides by Saccharomyces cerevisiae

被引:13
|
作者
Chung, Dong-Min [1 ]
Chung, Yung-Chul [2 ]
Maeng, Pil Je [3 ]
Chun, Hyo-Kon [1 ]
机构
[1] Korea Res Inst Biosci & Biotechnol, Microbe Based Fus Technol Res Ctr, Jeonbuk Branch Inst, Jeonbuk 580185, South Korea
[2] Int Univ Korea, Dept Food Nutr, Gyeongsang Namdo 660750, Jinju, South Korea
[3] Chungnam Natl Univ, Dept Microbiol, Taejon 305764, South Korea
关键词
Bioconversion; Isoquercitrin; Onion; Quercetin; Saccharomyces cerevisiae; ALLIUM-CEPA; GLYCOSIDES;
D O I
10.1007/s10529-010-0501-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioconversion of quercetin glucosides using four generally recognized as safe (GRAS) organisms (Aspergillus oryzae, Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) was evaluated by measuring changes in the levels of quercetin compounds of onion. Of the four organisms, S. cerevisiae increased the content of quercetin-3-O-beta-d-glucoside (III; isoquercitrin) and quercetin (IV), whereas decreasing quercetin-3,4'-O-beta-d-glucoside (I) and quercetin-4'-O-beta-d-glucoside (II). Also, S. cerevisiae converted authentic compound I to III, and II to IV, respectively. These results suggest that S. cerevisiae can be used to increase the levels of isoquercitrin (III), the most bioavailable quercetin compound in onion.
引用
收藏
页码:783 / 786
页数:4
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