Determination of Chlorophenols in Leathers and Textiles by Accelerated Solvent Extraction and High Performance Liquid Chromatography

A method was developed for the determination of chlorophenols (tetrachlorophenol and pentachlorophenol) in leathers and textiles by accelerated solvent extraction (ASE) and high performance liquid chromatography with diode array detection (HPLC/DAD). The crushed samples were extracted using ASE with n-hexane, and then the chlorophenols were eluted on a C(18) column with acetonitrile and 0.5% acetic acid solution, which were detected by DAD at lambda(214 nm). Qualitative and quantitative analysis was carried out by retention time of chlorophenols and external standard method. The mass concentration of tetrachlorophenol and pentachlorophenol has a linear range of 0. 1 - 20 mg/L with good correlation coefficients (r > 0. 9999); The mean recoveries were 96. 0% - 101. 5%, with the relative standard deviation (RSD) of 0. 50% - 5. 5% (n = 7), and the detection limit of this method for chlorophenols was 0. 02 mg/kg.