Unzipping of Double-Stranded DNA in Engineered α-Hemolysin Pores

被引：33

作者：

Liu, Aihua ^{[1
,2
]}

Zhao, Qitao ^{[1
]}

Krishantha, D. M. Milan ^{[1
]}

Guan, Xiyun ^{[1
]}

机构：

[1] Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA

[2] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Nanobioelect & Biosensors, Qingdao 266101, Peoples R China

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2011年 / 2卷 / 12期

基金：

美国国家卫生研究院;

关键词：

STOCHASTIC DETECTION; POLYNUCLEOTIDE MOLECULES; PROTEIN PORES; NANOPORE; TRANSLOCATION; DISCRIMINATION; ANALYTES;

D O I：

10.1021/jz200525v

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The biological protein a.-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the alpha-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the alpha-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing.

引用

页码：1372 / 1376

页数：5

共 50 条

[1] Shear unzipping of double-stranded DNA
Prakash, Shikha
Singh, Yashwant
[J]. PHYSICAL REVIEW E, 2011, 84 (03):
[2] Unzipping kinetics of double-stranded DNA in a nanopore
Sauer-Budge, AF
Nyamwanda, JA
Lubensky, DK
Branton, D
[J]. PHYSICAL REVIEW LETTERS, 2003, 90 (23)
[3] Measurements of the hysteresis in unzipping and rezipping double-stranded DNA
Hatch, K.
Danilowicz, C.
Coljee, V.
Prentiss, M.
[J]. PHYSICAL REVIEW E, 2007, 75 (05):
[4] What governs the unzipping process of double-stranded DNA
Wang Xiao-Feng
Lei Xiao-Ling
Fang Hai-Ping
[J]. CHINESE PHYSICS LETTERS, 2006, 23 (05) : 1339 - 1342
[5] ON A DISPERSIVE MODEL FOR THE UNZIPPING OF DOUBLE-STRANDED DNA MOLECULES
Calvo, J.
Nieto, J.
Soler, J.
Vasquez, M. O.
[J]. MATHEMATICAL MODELS & METHODS IN APPLIED SCIENCES, 2014, 24 (03): : 495 - 511
[6] Unzipping double-stranded DNA with a force: Numerical results
Chen, JZY
[J]. PHYSICAL REVIEW E, 2002, 66 (03):
[7] Unzipping of a double-stranded block copolymer DNA by a periodic force
Yadav, Ramu Kumar
Kapri, Rajeev
[J]. PHYSICAL REVIEW E, 2021, 103 (01)
[8] Capture and analysis of double-stranded DNA with the α-hemolysin nanopore: Fundamentals and applications
Denuga, Shekemi
Whelan, Donal E.
O'Neill, Shane P.
Johnson, Robert P.
[J]. ELECTROCHEMICAL SCIENCE ADVANCES, 2022, 2 (05):
[9] Mechanically Unzipping Double-Stranded DNA with Built-In Sequence Inhomogeneities and Bound Proteins
Lu, Ping
Sarkar, Abhijit
[J]. BIOPHYSICAL JOURNAL, 2011, 100 (03) : 153 - 153
[10] Overstretching Double-Stranded RNA, Double-Stranded DNA, and RNA-DNA Duplexes
Melkonyan, Lena
Bercy, Mathilde
Bizebard, Thierry
Bockelmann, Ulrich
[J]. BIOPHYSICAL JOURNAL, 2019, 117 (03) : 509 - 519

← 1 2 3 4 5 →