Electrospinning of in situ crosslinked recombinant human collagen peptide/chitosan nanofibers for wound healing

被引:69
|
作者
Deng, Aipeng [1 ]
Yang, Yang [2 ]
Du, Shimei [1 ]
Yang, Shulin [1 ]
机构
[1] Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China
[2] Univ Nottingham, Fac Engn, Nottingham NG7 2RD, England
基金
国家高技术研究发展计划(863计划);
关键词
SKIN REGENERATION; DRUG-DELIVERY; TGF-BETA; DIFFERENTIATION; PROLIFERATION; KERATINOCYTES; ANGIOGENESIS; ARCHITECTURE; SCAFFOLDS; DRESSINGS;
D O I
10.1039/c8bm00492g
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Electrospun collagen nanofibers are effective for wound healing; however, many problems, such as the tedious preparation process, weak strength and poor structure integration, limit further applications. In this study, recombinant human collagen (RHC) peptides and a simple one-step crosslinking strategy were used to prepare RHC/chitosan nanofibers. With the nonpathogenic, water-soluble RHC and a mild electrospinning solvent, in situ crosslinked nanofibers (S-CN) not only simplified the preparation procedure but also maintained a more integrated morphology. Compared with the immersed crosslinked nanofibers (I-CN), S-CN showed better performance in moisture retention, degradation and mechanical strength tests. In vitro cell proliferation, morphology and RT-PCR studies confirmed that fibroblasts presented better activities on nanofibers crosslinked in situ. Importantly, after treating with the nanofibers, rapid epidermidalization and angiogenesis were observed in an SD rat scalding model. All these data suggest that electrospun RHC/chitosan nanofibers crosslinked in situ are an ideal candidate that can be used for wound healing applications.
引用
收藏
页码:2197 / 2208
页数:12
相关论文
共 50 条
  • [1] Electrospinning of in situ crosslinked collagen nanofibers
    Meng, Linghui
    Arnoult, Oliver
    Smith, Meghan
    Wnek, Gary E.
    [J]. JOURNAL OF MATERIALS CHEMISTRY, 2012, 22 (37) : 19412 - 19417
  • [2] Alternating Current Electrospinning of Polycaprolactone/Chitosan Nanofibers for Wound Healing Applications
    del Olmo, Jon Andrade
    Mikes, Petr
    Asatiani, Nikifor
    Alonso, Jose Maria
    Saez Martinez, Virginia
    Perez Gonzalez, Raul
    [J]. POLYMERS, 2024, 16 (10)
  • [3] Preparation of a recombinant collagen-peptide (RHC)-conjugated chitosan thermosensitive hydrogel for wound healing
    Deng, Aipeng
    Yang, Yang
    Du, Shimei
    Yang, Xuanxin
    Pang, Shucai
    Wang, Xiaojie
    Yang, Shulin
    [J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2021, 119
  • [4] NOVEL RECOMBINANT HUMAN COLLAGEN FOR WOUND HEALING
    Shilo, Shani
    Dgany, Or
    Rosental, Michal
    Amir, Rachel
    Tal, Tamar
    Yaari, Amit
    Avraham, Tal
    Stein, Hanan
    Ofir, Keren
    Kredy-Farhan, Lily
    Ziv, Vered
    Amitai, Hagit
    Lapidot, Noa
    Shoseyov, Oded
    [J]. WOUND REPAIR AND REGENERATION, 2009, 17 (02) : A15 - A15
  • [5] Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing
    Rho, KS
    Jeong, L
    Lee, G
    Seo, BM
    Park, YJ
    Hong, SD
    Roh, S
    Cho, JJ
    Park, WH
    Min, BM
    [J]. BIOMATERIALS, 2006, 27 (08) : 1452 - 1461
  • [6] Chitosan-Collagen Hydrogel Modified with QHREDGS Peptide for Wound Healing
    Xiao, Y.
    Radisic, M.
    [J]. TISSUE ENGINEERING PART A, 2014, 20 : S102 - S102
  • [7] Injectable hydrogels of recombinant human collagen type III and chitosan with antibacterial and antioxidative activities for wound healing
    Xiong, Li
    Zhou, Chen
    Tong, Lei
    Han, Xiaowen
    Zou, Yaping
    Dong, Zuqin
    Liang, Jie
    Chen, Yafang
    Fan, Yujiang
    [J]. JOURNAL OF MATERIALS CHEMISTRY B, 2023, 11 (18) : 4131 - 4142
  • [8] Characterization of Electrospinning Chitosan Nanofibers Used for Wound Dressing
    Ali, Shahla H.
    Mahammed, Manaf A.
    Yasin, Suhad A.
    [J]. POLYMERS, 2024, 16 (14)
  • [9] Acceleration of diabetic wound healing with chitosan-crosslinked collagen sponge containing recombinant human acidic fibroblast growth factor in healing-impaired STZ diabetic rats
    Wang, Wei
    Lin, Shaoqiang
    Xiao, Yechen
    Huang, Yadong
    Tan, Yi
    Cai, Lu
    Li, Xiaokun
    [J]. LIFE SCIENCES, 2008, 82 (3-4) : 190 - 204
  • [10] Comparative properties of crosslinked electrospun collagen/chitosan nanofibers
    Xu Xiao-hong
    Gao Cai-Jiu
    Zhang Peng-yun
    Mo Xiu-mei
    He Chuang-long
    [J]. 2008 INTERNATIONAL SYMPOSIUM ON FIBER BASED SCAFFOLDS FOR TISSUE ENGINEERING, PROCEEDINGS, 2008, : 109 - 112