Green fluorescent protein: its application to the study of intracellular dynamics of steroid receptors

被引:1
|
作者
Georget, V [1 ]
Nicolas, JC [1 ]
Sultan, C [1 ]
机构
[1] INSERM, U439, F-34090 Montpellier, France
来源
M S-MEDECINE SCIENCES | 1999年 / 15卷 / 01期
关键词
D O I
10.4267/10608/1195
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The green fluorescent protein (GFP) comes from the Aequorea victoria jellyfish. In vivo, it acquires the capacity of fluorescence by activation of aequorin. In vitro, it is activated by simple exposure to standard long-wave ultraviolet or blue light. GFP does not require the addition of substrate or cofactors for generation of its green light and it represents a new tool for monitoring the dynamic processes in living cells and organisms. It can produce fluorescence in I various organisms, such as bacteria, plants and mammals. GFP is now applied as a reporter for gene expression, as a marker to study cell lineage and as a tag to localize proteins in living cells. GFP does not require cell fixation or other chemical steps for analysis, contrary to immunocytochemistry. Immunodetection needs fixation and permeabilization steps, which may lead to artefacts in the pattern of localization. This would explain particularly the contradictory results about the localization of the steroid receptors. Several studies have thus been developed using GFP tagged to different steroid receptors: glucocorticoid receptor (GR), mineralocorticoid receptor (MR) and androgen receptor (AR). In absence of hormone, GFP-GR and GFP-AR were described to be cytoplasmic, while the GFP-MR was both cytoplasmic and nuclear. In presence of each specific ligand, GFP-steroid receptors were all nuclear. Formation of nuclear clusters were observed in the presence of agonists for GFP-GR and GFP-MR. GFP permits the analysis of dynamic cellular events and has already been established as an important tool for many branches of biological research.
引用
收藏
页码:45 / 55
页数:11
相关论文
共 50 条
  • [1] Direct visualization of intracellular trafficking of GRP receptors using green fluorescent protein
    Yee, HF
    Walsh, JH
    GASTROENTEROLOGY, 1997, 112 (04) : A1190 - A1190
  • [2] Review -: Using green fluorescent protein to study intracellular signalling
    Tavaré, JM
    Fletcher, LM
    Welsh, GI
    JOURNAL OF ENDOCRINOLOGY, 2001, 170 (02) : 297 - 306
  • [3] Green fluorescent protein as a probe to study intracellular solute diffusion
    Verkman, AS
    GREEN FLUORESCENT PROTEIN, 1999, 302 : 250 - 264
  • [4] Synthesis of green fluorescent protein-ricin and monitoring of its intracellular trafficking
    Department of Surgery, Medical University of South Carolina, Charleston, SC 29425, United States
    不详
    不详
    不详
    不详
    Bioconjugate Chemistry, 8 (05): : 743 - 750
  • [5] Synthesis of green fluorescent protein-ricin and monitoring of its intracellular trafficking
    Tagge, E
    Harris, B
    Burbage, C
    Hall, P
    Vesely, J
    Willingham, M
    Frankel, A
    BIOCONJUGATE CHEMISTRY, 1997, 8 (05) : 743 - 750
  • [7] Studies on the structure, mechanism of green fluorescent protein and its application
    Liu, Zu-qiang
    Hu, Min
    Qi, Yi-peng
    Wuhan Daxue Xuebao/Journal of Wuhan University, 2000, 46 (02): : 211 - 214
  • [8] Ultrafast excited state dynamics of the green fluorescent protein chromophore and its kindling fluorescent protein analogue
    Addison, Kiri
    Heisler, Ismael A.
    Conyard, Jamie
    Dixon, Tara
    Page, Philip C. Bulman
    Meech, Stephen R.
    FARADAY DISCUSSIONS, 2013, 163 : 277 - 296
  • [9] Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein
    Milligan, G
    BRITISH JOURNAL OF PHARMACOLOGY, 1999, 128 (03) : 501 - 510
  • [10] Application of a chimeric green fluorescent protein to study protein-protein interactions
    Garamszegi, N
    Garamszegi, ZP
    Rogers, MS
    DeMarco, SJ
    Strehler, EE
    BIOTECHNIQUES, 1997, 23 (05) : 864 - +