Microsatellite Fingerprinting of Maize Cultivars (Zea mays L.)

被引:2
|
作者
Kaur, H. [1 ]
Sarao, N. K. [1 ]
Vikal, Y. [1 ]
Singh, K. [1 ]
Sharma, R. C. [1 ]
机构
[1] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana 141004, Punjab, India
关键词
Zea mays; maize; molecular characterization; SSR markers; DNA fingerprinting; GENETIC DIVERSITY; DNA; SEQUENCE; MARKERS; UTILITY;
D O I
10.1556/CRC.39.2011.4.5
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Maize cultivars (4 composites, 14 inbreds and 7 hybrids) cultivated in Punjab were characterized using a set of 70 Simple Sequence Repeat (SSR) markers with a total of 199 alleles. Out of these, 67 markers produced polymorphic profiles, 3 were monomorphic. Eight SSR primers detected eight unique alleles in three genotypes. These unique SSR alleles provide an opportunity for unambiguous differentiation of the respective genotypes. SSR profiles were analyzed by using computer softwares NTSYS-pc and DARwin 5.0. Both of them classified the genotypes in four distinct groups.
引用
收藏
页码:507 / 514
页数:8
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