Microbial phylogeny and diversity: Small subunit ribosomal RNA sequence analysis and beyond

被引:162
|
作者
Rajendhran, J. [1 ]
Gunasekaran, P. [1 ]
机构
[1] Madurai Kamaraj Univ, Sch Biol Sci, Dept Genet, Ctr Excellence Genom Sci, Madurai 625021, Tamil Nadu, India
关键词
16S rRNA; Phylogeny; Diversity; Metagenomics; Multilocus sequence analysis; GRADIENT GEL-ELECTROPHORESIS; BACTERIAL SYSTEMATICS; POLYPHASIC TAXONOMY; SPECIES DEFINITION; DNA-POLYMERASE; GENES; PCR; GENUS; SOIL; CLASSIFICATION;
D O I
10.1016/j.micres.2010.02.003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Small subunit ribosomal RNA (16S rRNA) gene sequence analysis is used for the identification and classification of prokaryotes. In addition, sequencing of 16S rRNA genes amplified directly from the environment is used to estimate microbial diversity. The presence of mosaicism, intra-genonnic heterogeneity and the lack of a universal threshold sequence identity value limit 16S rRNA-based phylogenetic analysis. PCR-amplification bias and cloning bias can also result in an inaccurate representation of the microbial diversity. In this review, recently reported complexities of 16S rRNA gene sequence analyses and the requirement of additional tools for microbial phylogeny and diversity analyses are discussed. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:99 / 110
页数:12
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