Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil

被引:0
|
作者
Reck, Carolina [1 ,2 ]
Menin, Alvaro [3 ]
Batista, Franciane [1 ]
Santos, Patricia Oliveira Meira [4 ]
Miletti, Luiz Claudio [1 ]
机构
[1] Univ Estado Santa Catarina, Ctr Ciencias Agrovet, Dept Prod Anim & Alimentos, Lab Bioquim Hemoparasitas & Vetores, Av Luiz Camoes 2090, Lages, SC, Brazil
[2] Inst Vet Res & Diagnost, VERTA Lab Vet Diagnost, Av Lions 1286, BR-89520000 Curitibanos, SC, Brazil
[3] Univ Fed Santa Catarina, Departmento Biociencias & Saude Unica, Rodovia Ulysses Gaboardi 3000,Km 3, BR-89520000 Curitibanos, SC, Brazil
[4] Univ Fed Sergipe, Ctr Ciencias Agr, Av Marechal Rondon,Cidade Univ, BR-49100000 Sao Cristovao, SE, Brazil
关键词
Trypanosomosis; Serodiagnosis; Haemoprotozoans; Epidemiological surveillance; IDENTIFICATION; INFECTION; BOVINES; ASSAY; PCR;
D O I
10.1016/j.crpvbd.2021.100024
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Surra is an infectious disease caused by Trypanosoma evansi, which affects a large number of domestic and wild animal species. Infection control is based on rapid diagnosis followed by treatment of sick animals. This study aimed to evaluate a buffered T. evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti -T. evansi antibodies in serum samples of horses. For this purpose, 445 serum samples from horses were evaluated and the results compared with the diagnosis by CATT/T. evansi. Our data show a sensitivity of 92%, specificity of 91% and a degree of agreement kappa (?) of 0.82 (95% CI: 0.771-0.877, P < 0.01) between BA/Te and CATT/ T. evansi. Antigen specificity was also evaluated against reactive serum for other infectious agents circulating in equine herds. In conclusion, our findings show that BA/Te has the potential to be a practical and quick screening method for the detection of anti -T. evansi antibodies in horses.
引用
收藏
页数:4
相关论文
共 8 条