Purification and Characterization of a Lectin from Chinese Quince (Chaenomeles sinensis) Fruit

被引：1

作者：

Ishiwari, Atsuya ^{[1
]}

Akutsu, Tomoko ^{[2
]}

Ikegaya, Hiroshi ^{[3
]}

Sakurada, Koichi ^{[2
]}

机构：

[1] Kanagawa Prefectural Police Headquarters, Sci Criminal Invest Lab, Naka Ku, Yokohama, Kanagawa 23123, Japan

[2] Natl Res Inst Police Sci, Kashiwa, Chiba 277882, Japan

[3] Kyoto Prefectural Univ Med, Kyoto 6020841, Japan

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 19卷 / 02期

关键词：

Chaenomeles sinensis; Chinese quince; N-acetyllactosamine-specific; lectin; MICROARRAY;

D O I：

10.1007/BF03263348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chaenomeles sinensis lectin (CSL) was isolated from Chinese quince fruit by affinity chromatography. The molecular weight of native CSL was estimated to be 16 kD by gel filtration chromatography. This lectin was found to contain approximately 57% carbohydrates. The molecular weight of deglycosylated CSL was estimated to be 3.6 kD by tricine-SDS-PAGE under reduced conditions. Our results suggest that CSL may be a homodimer. The hemagglutinating activity of CSL was inhibited by N-acetyllactosamine and chicken ovalbumin, and it was drastically decreased at pH levels of 9.0 or greater. CSL may be a useful tool for the purification of glycoconjugates.

引用

页码：243 / 245

页数：3

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