Response of N2O emission to water and nitrogen addition in temperate typical steppe soil in Inner Mongolia, China

The Chinese steppe is undergoing a drastic increase in nitrogen (N) deposition, and the precipitation in this region is predicted to increase. However, the response of soil N2O emissions to the coupling changes of precipitation and N deposition in grassland ecosystem has been seldom discussed. A manipulative field experiment was conducted to investigate the individual and interactive effects of precipitation increase and N deposition on soil N2O efflux in semi-arid temperate grassland in Inner Mongolia during the growing seasons of 2010 and 2011.The treatments included four N addition levels [20 g N m(-2) y(-1) (N20) 10 gN m(-2) y(-1) (N10), 5 g N m(-2) y(-1) (N5), and a zero-N control (CK)] and two water application levels [natural precipitation for dry (D) and 15% increase of long-term mean annual precipitation for wet (W) treatments]. Results indicated that N and water addition both significantly increased soil N2O effluxes < 0.01). The maximum N2O emissions were observed within 2-3 days after N addition to all treatments, and the N2O effluxes in W treatments were generally higher than in D treatments for the same N input level. For the treatments without N inputs, the N2O emission peak of WCK was 9.8% higher than DCK in 2010. The effect of water addition on N2O emission was more evident when more N fertilizer was applied. For the high N input treatments, the maximum N2O emission of WN20 treatment was 222.6% higher than DN20 in 2010. The changes in N and water availabilities accounted for 91.3% (2010) and 75.6% (2011) of the N2O cumulative efflux variation among different treatments (P < 0.01). The N2O effluxes were significantly affected by the interactive effect between N and water in 2010 and 2011 (P < 0.05). Significant interannual variability in the cumulative N2O emissions was observed, the cumulative N2O emissions in 2011 were significantly lower than those in 2010 even though the summer of 2011 experienced higher rainfall (P < 0.01). Temperature also significantly influenced soil N2O emission apart from the effects of water and N. The temperature change accounted for 41.3% (W) to 47.2% (D) of the temporal variations in N2O emission during the relatively dry 2010. The combined changes in soil moisture, NH4+-N, and temperature accounted for 43.1% (D) to 54.5% (W) of the temporal variations in N2O emission in the relatively wet year of 2011. (C) 2015 Elsevier B.V. All rights reserved.