Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy

被引：26
|
作者：
Moers, MHP [1 ]
Kalle, WHJ [1 ]
Ruiter, AGT [1 ]
Wiegant, JCAG [1 ]
Raap, AK [1 ]
Greve, J [1 ]
DeGrooth, BG [1 ]
VanHulst, NF [1 ]
机构：
[1] LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY,2333 AL LEIDEN,NETHERLANDS
来源：
JOURNAL OF MICROSCOPY-OXFORD | 1996年 / 182卷
关键词：
near-field scanning optical microscopy; fluorescence microscopy; fluorescence in situ hybridization; human metaphase chromosomes; DNA; sequencing;
D O I：
10.1111/j.1365-2818.1996.tb04795.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.
引用
收藏
页码：40 / 45
页数：6
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