FINGERPRINTING OF CORYNEBACTERIUM PSEUDOTUBERCULOSISSTRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA AND AMPLIFIED FRAGMENT LENGTH POLYMORPHISM TECHNIQUES

被引：0
|
作者：
Nowicki, Mariusz [1 ]
Kaba, Jaroslaw [1 ]
Mosiej, Ewa [2 ]
Lutynska, Anna [2 ]
Augustynowicz, Ewa [2 ]
Rzewuska, Magdalena
Binek, Marian
Szalus-Jordanow, Olga
Stefanska, Ilona [2 ]
Frymus, Tadeusz
机构：
[1] Warsaw Univ Life Sci, Div Infect Dis & Epidemiol, Dept Large Anim Dis Clin, Fac Vet Med, PL-02776 Warsaw, Poland
[2] Natl Inst Publ Hlth, Div Epidemiol, Dept Sera & Vaccine Evaluat, Natl Influenza Ctr, PL-00791 Warsaw, Poland
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2011年 / 55卷 / 03期
关键词：
goats; Corynebacterium pseudotuberculosis; caseous lymphadenitis; phenotyping; genotyping; CASEOUS-LYMPHADENITIS; STRAINS; AFLP; DIAGNOSIS; SHEEP; GOATS;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the study, phenotypic and genotypic properties of 42 Corynebacterium pseudotuberculosis strains isolated from 16 goat flocks were determined by random amplified polymorphic DNA and amplified fragment length polymorphism techniques. Several sets of RAPD primers and AFLP protocols were experimentally evaluated for potential of generating amplification profiles of the best discriminatory power. The overall similarity level of profiles by cluster analysis generated with RAPD3 (64%) set was higher than obtained for both RAPD 2 (70%) and AFLP (88%). The results confirmed usefulness of both AFLP and RAPD methods to discriminate strains of C. pseudotuberculosis, even at the flocks' level, and confirmed a single genotype as the most prevalent in goats coming from flocks in Poland.
引用
收藏
页码：403 / 409
页数：7
相关论文
共 50 条
  • [1] Estimating nucleotide diversity from random amplified polymorphic DNA and amplified fragment length polymorphism data
    Borowsky, RL
    [J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 2001, 18 (01) : 143 - 148
  • [2] DNA-FINGERPRINTING BY RANDOM AMPLIFIED POLYMORPHIC DNA AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IS USEFUL FOR YEAST TYPING
    PAFFETTI, D
    BARBERIO, C
    CASALONE, E
    CAVALIERI, D
    FANI, R
    FIA, G
    MORI, E
    POLSINELLI, M
    [J]. RESEARCH IN MICROBIOLOGY, 1995, 146 (07) : 587 - 594
  • [3] Amplified fragment length polymorphism versus random amplified polymorphic DNA markers:: clonal diversity in Saxifraga cernua
    Kjolner, S
    Såstad, SM
    Taberlet, P
    Brochmann, C
    [J]. MOLECULAR ECOLOGY, 2004, 13 (01) : 81 - 86
  • [4] Restriction fragment length polymorphism and random amplified polymorphic DNA analysis of chickpea accessions
    Banerjee, H
    Pai, RA
    Sharma, RP
    [J]. BIOLOGIA PLANTARUM, 1999, 42 (02) : 197 - 208
  • [5] Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers
    Bednarek, Piotr T.
    Masojc, Piotr
    Lewandowska, Renata
    Myskow, Beata
    [J]. JOURNAL OF APPLIED GENETICS, 2003, 44 (01) : 21 - 33
  • [6] Random amplified polymorphic DNA and amplified fragment length polymorphism assessment of genetic variation in Nicaraguan populations of Pinus oocarpa
    Díaz, V
    Muñiz, LM
    Ferrer, E
    [J]. MOLECULAR ECOLOGY, 2001, 10 (11) : 2593 - 2603
  • [7] Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting tor identification of Acinetobacter genomic species and typing of Acinetobacter baumannii
    Koeleman, JGM
    Stoof, J
    Biesmans, DJ
    Sabelkoul, PHM
    Vandenbroucke-Grauls, CMJE
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) : 2522 - 2529
  • [8] Amplified fragment length polymorphism markers for DNA fingerprinting in the genus Salvia
    Braglia, L.
    Casabianca, V.
    De Benedetti, L.
    Pecchioni, N.
    Mercuri, A.
    Cervelli, C.
    Ruffoni, B.
    [J]. PLANT BIOSYSTEMS, 2011, 145 (02): : 274 - 277
  • [9] In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants
    Watanabe, A
    Araki, S
    Kobari, S
    Sudo, H
    Tsuchida, T
    Uno, T
    Kosaka, N
    Shimomura, K
    Yamazaki, M
    Saito, K
    [J]. PLANT CELL REPORTS, 1998, 18 (3-4) : 187 - 192
  • [10] In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants
    A. Watanabe
    S. Araki
    S. Kobari
    H. Sudo
    T. Tsuchida
    T. Uno
    N. Kosaka
    K. Shimomura
    M. Yamazaki
    K. Saito
    [J]. Plant Cell Reports, 1998, 18 : 187 - 192
12345