In vitro tissue culture of the medicinal shrub Calotropis procera to produce pharmacologically active proteins from plant latex

被引：13

作者：

Teixeira, Fabian M. ^{[1
]}

Ramos, Marcio V. ^{[1
]}

Soares, Arlete A. ^{[2
]}

Oliveira, Raquel S. B. ^{[1
]}

Almeida-Filho, Luiz Carlos P. ^{[1
]}

Oliveira, Jefferson S. ^{[4
]}

Marinho-Filho, Jose D. B. ^{[3
]}

Carvalho, Cristina Paiva S. ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60430270 Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Biol, BR-60430270 Fortaleza, Ceara, Brazil

[3] Univ Fed Ceara, Dept Fisiol & Farmacol, Fac Med, BR-60430270 Fortaleza, Ceara, Brazil

[4] Univ Fed Piaui, Dept Ciencias Saude, Parnaiba, Piaui, Brazil

来源：

PROCESS BIOCHEMISTRY | 2011年 / 46卷 / 05期

关键词：

Bioresources; Dengue; Callus; Latex proteins; Pharmacological tools; NITRIC-OXIDE; INFLAMMATION; PERITONITIS; INVOLVEMENT; GROWTH;

D O I：

10.1016/j.procbio.2011.01.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The latex of Calotropis procera is a rich source of proteins that have anti-inflammatory, anti-nociceptive and selective cytotoxic and anti-tumorigenic properties. In this study, two distinct protocols were developed to culture C. procera in vitro in order to obtain active molecules. Soluble proteins of both callus and root cultures were extracted and tested for various activities found in the latex. Anti-inflammatory and anti-nociceptive proteins were present in callus and root extracts; however, these proteins did not possess cytotoxic or anti-tumorigenic activity. Larvicidal proteins were present, but they were not related to others that have been reported previously in latex. This study confirms that tissue culture of C. procera is able to produce therapeutic-grade proteins that have the potential to relieve inflammation and pain associated with inflammatory disorders. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1118 / 1124

页数：7

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