Id1 Interacts and Stabilizes the Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) in Nasopharyngeal Epithelial Cells

被引:15
|
作者
Hau, Pok Man [1 ]
Tsang, Chi Man [1 ]
Yip, Yim Ling [1 ]
Huen, Michael S. Y. [2 ,3 ]
Tsao, Sai Wah [1 ]
机构
[1] Univ Hong Kong, Dept Anat, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Dept Anat, Genome Stabil Res Lab, Hong Kong, Hong Kong, Peoples R China
[3] Univ Hong Kong, Ctr Canc Res, Hong Kong, Hong Kong, Peoples R China
来源
PLOS ONE | 2011年 / 6卷 / 06期
关键词
NF-KAPPA-B; LINES HNE-1; EXPRESSION; GROWTH; MEMBRANE-PROTEIN-1; LOOP; ESTABLISHMENT; CARCINOMA; ACTIVATION; PATHWAYS;
D O I
10.1371/journal.pone.0021176
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The EBV-encoded latent membrane protein 1 (LMP1) functions as a constitutive active form of tumor necrosis factor receptor (TNFR) and activates multiple downstream signaling pathways similar to CD40 signaling in a ligand-independent manner. LMP1 expression in EBV-infected cells has been postulated to play an important role in pathogenesis of nasopharyngeal carcinoma. However, variable levels of LMP1 expression were detected in nasopharyngeal carcinoma. At present, the regulation of LMP1 levels in nasopharyngeal carcinoma is poorly understood. Here we show that LMP1 mRNAs are transcribed in an EBV-positive nasopharyngeal carcinoma (NPC) cell line (C666-1) and other EBV-negative nasopharyngeal carcinoma cells stably re-infected with EBV. The protein levels of LMP1 could readily be detected after incubation with proteasome inhibitor, MG132 suggesting that LMP1 protein is rapidly degraded via proteasome-mediated proteolysis. Interestingly, we observed that Id1 overexpression could stabilize LMP1 protein in EBV-infected cells. In contrary, Id1 knockdown significantly reduced LMP1 levels in cells. Co-immunoprecipitation studies revealed that Id1 interacts with LMP1 by binding to the CTAR1 domain of LMP1. N-terminal region of Id1 is required for the interaction with LMP1. Furthermore, binding of Id1 to LMP1 suppressed polyubiquitination of LMP1 and may be involved in stabilization of LMP1 in EBV-infected nasopharyngeal epithelial cells.
引用
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页数:10
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