Identification of Drug-Type and Fiber-Type of Hemp (Cannabis sativa L.) by Multiplex PCR

被引：0
|
作者：
Thichak, Somkhid [1 ]
Natakankitkul, Surapol [2 ]
Chansakaow, Sunee [2 ]
Chutipongvivate, Salakchit [3 ]
机构：
[1] Reg Med Sci Ctr 10, Chiang Mai 50180, Thailand
[2] Chiang Mai Univ, Fac Pharm, Dept Pharmaceut Sci, Chiang Mai 50200, Thailand
[3] Reg Med Sci Ctr 3, Chon Buri 20000, Thailand
来源：
CHIANG MAI JOURNAL OF SCIENCE | 2011年 / 38卷 / 04期
关键词：
hemp; Cannabis sativa L; multiplex PCR; molecular marker; ACID SYNTHASE; GENE; CHEMOTYPE;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The multiplex PCR method for identification of drug type and fiber-type of 100 hemp samples was developed and evaluated. Two loci multiplex PCR molecular markers were simultaneously amplified and detected. The first locus, 700 bp-Cannabis marker, was designed to be Cannabis genus specific, while the second locus, 1.2 kb-drug-type marker, was specific to THCA synthase gene of drug-type plants. The results showed that Cannabis marker was detected in all samples, but drug-type marker was found in only 63 samples. Therefore, by this method, 37 plants could be identified to be fiber-type, whereas the other plants were considered as drug-type. The relative accuracy between the developed multiplex PCR method and reference method (based on CBD/THC ratio) was 93%.
引用
收藏
页码：608 / 618
页数：11
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