Localization microscopy at doubled precision with patterned illumination

被引：134
|
作者：
Cnossen, Jelmer [1 ,2 ]
Hinsdale, Taylor [1 ]
Thorsen, Rasmus O. [1 ]
Siemons, Marijn [3 ]
Schueder, Florian [4 ,5 ,6 ]
Jungmann, Ralf [4 ,5 ,6 ]
Smith, Carlas S. [1 ,2 ,7 ]
Rieger, Bernd [1 ]
Stallinga, Sjoerd [1 ]
机构：
[1] Delft Univ Technol, Dept Imaging Phys, Delft, Netherlands
[2] Delft Univ Technol, Delft Ctr Syst & Control, Delft, Netherlands
[3] Univ Utrecht, Dept Biol, Utrecht, Netherlands
[4] Ludwig Maximilians Univ Munchen, Dept Phys, Munich, Germany
[5] Ludwig Maximilians Univ Munchen, Ctr Nanosci, Munich, Germany
[6] Max Planck Inst Biochem, Martinsried, Germany
[7] Univ Oxford, Dept Engn Sci, Oxford, England
来源：
NATURE METHODS | 2020年 / 17卷 / 01期
基金：
美国国家卫生研究院;
关键词：
SINGLE-MOLECULE LOCALIZATION; SUPERRESOLUTION MICROSCOPY; RESOLUTION; NANOSCOPY; TRACKING;
D O I：
10.1038/s41592-019-0657-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging. SIMFLUX combines elements of MINFLUX with structured illumination to double localization precision and improve resolution in localization microscopy. The approach was demonstrated on DNA origami and on cellular microtubules.
引用
收藏
页码：59 / +
页数：9
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