Effect of transforming growth factor-β on calcium homeostasis in prostate carcinoma cells

Ca2- plays a fundamental role in the control of a variety of cellular functions, in particular, in energy metabolism and apoptosis. In this study. we show that TGF-beta at concentrations of 0.1-1.0 ng/ml transiently increases the level of intracellular Ca2+ ([Ca2+](in)) in human prostate carcinoma. PC-3U, cells. Experiments with mitochondrial inhibitors (oligomycin and antimycin A) and an inhibitor of endoplasmic reticulum Ca2+ uptake (BHQ) implied that the effect of TGF-beta1 was due to an effect on the mitochondria. TGF-beta1 treatment resulted in a decrease in ATP synthesis and to a depolarisation, leading to a release of Ca2+ from mitochondria and decreased activity of the Ca2+ pumps. Analysis of the mitochondria within the PC-3U cells by polarography and membrane potential-sensitive dye (Rhodamine 123) confirmed that under these experimental conditions, TGF-beta1 inhibited ATP synthesis and depolarised the mitochondria. The results implicate that TGF-beta1 affects the function of the mitochondria and may be of significance for the understanding of the proapoptotic effect of TGF-beta1 in these cells. (C) 2003 Elsevier Science (USA). All rights reserved.