Enhancement of antimicrobial and antimutagenic activities of Korean barberry (Berberis koreana Palib.) by the combined process of high-pressure extraction with probiotic fermentation

BACKGROUND: To evaluate the combined effects of high pressure extraction (HPE) and probiotic fermentation on the antimicrobial and antimutagenic activities, Berberis koreana was subjected to 500 MPa for 30 min and then fermented with Bifidobacterium longum B6 (HPE-BLF) and Lactobacillus paracasei (HPE-LPF) at 37 degrees C for 6 days. RESULTS: The phenol content was significantly increased to 228 mg GAE g(-1) by the HPE compared to the conventional extraction (CE, 188 mg GAE g(-1)). The HPE-BLF and HPE-LPF showed the highest antimicrobial activity (MIC < 4 mg mL(-1)) against beta-lactam antibiotic sensitive and resistant Staphylococcus aureus. No significant mutagenic effect was observed for CE, HPE, HPE-BLF, and HPE-LPF extracts. The highest antimutagenic activities against frame-shift mutant Salmonella typhimurium were observed at the HPE-LPF (82%), followed by the HPE-BLF (77%). CONCLUSION: The combined HPE and fermentation process could be used as an alternative extraction method for improving the extraction efficacy of medicinal plants. The results will provide pharmaceutically useful information and potential direction for finding new drug sources from medicinal plants. (c) 2010 Society of Chemical Industry