Comparison of Cellular Transforming Activity of OCT4, NANOG, and SOX2 in Immortalized Astrocytes

被引：8
|
作者：
Seo, Sunyoung [1 ]
Jeon, Hee-Young [1 ,2 ]
Kim, Hyunggee [1 ,2 ]
机构：
[1] Korea Univ, Dept Biotechnol, Coll Life Sci & Biotechnol, Seoul 02841, South Korea
[2] Korea Univ, Inst Anim Mol Biotechnol, Seoul, South Korea
来源：
DNA AND CELL BIOLOGY | 2017年 / 36卷 / 11期
基金：
新加坡国家研究基金会;
关键词：
astrocytes; cellular transformation; NANOG; NFB; OCT4; SOX2; EMBRYONIC STEM-CELLS; HUMAN GLIOMAS; CANCER; EXPRESSION; LINES; DIFFERENTIATION; PHENOTYPE; UPDATE; TUMORS; GENE;
D O I：
10.1089/dna.2017.3889
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Embryonic stem cell factorsOCT4, NANOG, and SOX2contribute to the maintenance of stem cell properties and malignant progression in various cancers, including glioblastoma. Although functional roles of each of these genes are well documented in stem cell and cancer biology, no study has directly compared their cellular transforming activity under same experimental conditions. In this study, we compared the cellular transforming activity of OCT4, NANOG, and SOX2 using human immortalized astrocytes cultured under serum-free stem cell culture conditions. We found that SOX2 exhibited the strongest transforming activities, such as cell proliferation, neurosphere formation, resistance to cytotoxic drug, and cell migration/invasion, which may be associated with the activation of the nuclear factor kappa B (NFB) signaling pathway. Thus, OCT4, NANOG, and SOX2, known to be frequently activated in various cancers and cancer stem cells, may play a distinct role in the regulation of cellular transformation.
引用
收藏
页码：1000 / 1009
页数：10
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