Paper-based Platform for Urinary Creatinine Detection

A new paper platform was developed for the colorimetric detection of creatinine. The filter paper was coated with 3-propylsulfonic acid trimethoxysilane and used as the platform. Creatinine in a cationic form was extracted onto the paper via an ion-exchange mechanism and detected through the Jaffe reaction, resulting in a yellow-orange color complex. The color change on the paper could be observed visually, and the quantitative detection of creatinine was achieved through monitoring the color intensity change. The color intensity of creatinine complexes on the paper platform as a function of the creatinine concentration provided a linear range for creatinine detection in the range of 10 - 60 mg L-1 and a detection limit of 4.2 mg L-1. The accuracy of the proposed paper-based method was comparable to the conventional standard Jaffe method. This paper platform could be applied for simple and rapid detection of creatinine in human urine samples with a low consumption of reagent.