Immobilizing Enzyme Biocatalysts onto Porous Polymer Monoliths Prepared from Cocontinuous Polymer Blends

被引:4
|
作者
Liberelle, Benoit [1 ]
Dil, Ebrahim Jalali [2 ]
Sabri, Faezeh [2 ]
Favis, Basil D. [2 ]
De Crescenzo, Gregory [1 ]
Virgilio, Nick [2 ]
机构
[1] Polytech Montreal, Dept Chem Engn, Montreal, PQ H3C 3A7, Canada
[2] Polytech Montreal, Res Ctr High Performance Polymer & Composite Syst, Dept Chem Engn, Montreal, PQ H3C 3A7, Canada
关键词
cocontinuous polymer blend; enzyme; porosity; surface modification; biocatalysis; monolith; nanoparticles; GLUCOSE-OXIDASE; NANO-SILICA; MORPHOLOGY; NANOPARTICLES; LOCALIZATION;
D O I
10.1021/acsapm.1c01144
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
We demonstrate how macroporous poly(epsilon-caprolactone) monoliths, prepared from cocontinuous polymer blends, can be used to immobilize enzyme biocatalysts.herein demonstrated with glucose oxidase (GOx) and peroxidase (POx). The pores are covered with a self-assembled layer of aminated sub-mu m silica particles, over which a thin, low-fouling carboxymethylated dextran (CMD) layer is grafted. GOx or POx is finally grafted onto the CMD layer. Our materials display a combination of strong enzyme immobilization, low nonspecific adsorption, and significant enzymatic activity. This approach constitutes a versatile technological platform for the immobilization of biomolecules onto polymer-based monoliths with precisely controlled porosity parameters for both batch and continuous-flow bioprocesses.
引用
收藏
页码:6359 / 6365
页数:7
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