This paper from the perspective of business partners and industry characteristics, researches the 224 companies listed on the board with the background of venture capital of Shenzhen 2009-2016, constructs the performance indicators of venture investment from two aspects, one is output level, another is benefit level. Using empirical test studies the influence on venture capital investment performance in the role of venture partners and industry characteristics. Research shows that: In the economic background of listed companies, venture capital partners effect showed negative influence; Associate degree of partners and the characteristics of the industry will have a significant negative correlation on venture capital investment performance, but in the field of new and high technology industry, the selection of certain characteristics of the industry positively have active influence on the benefit level performance of venture capital investment; The holding time of venture capital project has a significantly positive effects on the output level of venture capital investment performance.