Cloning and characterization of another lignin peroxidase gene from the white-rot fungus Phanerochaete chrysosporium

Lignin peroxidase (LIP) isozymes of Phanerochaete chrysosporium are encoded by a large family of closely related genes, whose total number is still unknown. Among genomic clones, obtained using the polymerase chain reaction to clone the LIP gene LPOA from Phanerochaefe chrysosporium strain BKM-F 1767, another LIP gene was found. This gene, HG3, showed more than 95% nucleotide homology to those LIP gene variants which encode LIP isozyme H8. The gene encodes a protein of 372 amino acids, including the typical leader sequence for secretion, that is identical to the LIP isozyme H8 except for 6 amino acid substitutions.