Involvement of intracellular Ca2+ in the regulatory volume decrease after hyposmotic swelling in MDCK cells

被引：5

作者：

Iida-Tanaka, Naoko

Namekata, Iyuki

Kaneko, Megumi

Tamura, Miku

Kawanishi, Toru

Nakamura, Ryu

Shigenobu, Koki

Tanaka, Hikaru ^{[1
]}

机构：

[1] Toho Univ, Fac Pharmaceut Sci, Dept Pharmacol, Funabashi, Chiba 2748510, Japan

[2] Otsuma Womans Univ, Fac Home Econ, Dept Food Sci, Tokyo 1028357, Japan

[3] Natl Inst Hlth Sci, Div Drugs, Setagaya Ku, Tokyo 1588501, Japan

[4] Prod Management Div Microscopy, Adv Imaging Microscopy Dept, Shinjuku Ku, Tokyo 1600003, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2007年 / 104卷 / 04期

关键词：

hyposmosis; cell volume; intracellular Ca2+;

D O I：

10.1254/jphs.SC0070024

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We examined the source of Ca 21 involved in the volume regulation of Madin-Darby canine kidney (MDCK) cells with confocal microscopy and fluoroprobes. Hyposmosis induced a transient increase in cell volume, as well as cytoplasmic Ca2+, which peaked at 3 to 5 min and gradually decreased to reach the initial value within about 30 min. This late decrease in cell volume, as well as the transient rise in cytoplasmic Ca2+, was reduced in Ca2+-free solution and was abolished by pretreatment with thapsigargin. In conclusion, Ca2+ released from the intracellular store contributes to the regulatory volume decrease following hyposmotic swelling in MDCK cells.

引用

页码：397 / 401

页数：5

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