Media and genotype effects on the development and conversion of somatic alfalfa (Medicago sativa L.) embryos

被引：0

作者：

Hindson, S

McElroy, AR

Portelance, C

机构：

[1] Agr & Agri Food Canada, ECORC, Ottawa, ON K1A 0C6, Canada

[2] Agr & Agri Food Canada, PARC, Summerland, BC, Canada

[3] Canadian Food Inspect Agcyt, Plant Prod Div, Nepean, ON K1A 0Y9, Canada

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 1998年 / 34卷 / 03期

关键词：

alfalfa; Medicago sativa; somatic embryogenesis;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Various preconditioning treatments of alfalfa (Medicago sativa L.) somatic embryos to improve embryo quality and conversion were studied. Four different regenerating genotypes were compared. Embryogenic cultures were established in liquid culture. Globular embryos were collected and plated on an embryo development medium until they reached cotyledonary stage. They were then exposed to three treatments: a standard embryo development medium (control), media supplementation with 1 mu M abscisic acid (ABA), 50 mM glutamine and 5% sucrose (T), additional supplementation with 50 mu M ABA (TT), and additional supplementation followed by desiccation (TTD). Treatments affected embryo conversion, but not uniformly for all genotypes. Embryo conversion was increased (P < 0.05) by pretreatment (T), while only one exhibited any response to additional ABA (T vs. TT). Desiccation decreased (P < 0.05) conversion of pretreated embryos (TT vs. TTD) of all genotypes. The effect of treatments on plantlet weight was less pronounced and inconsistent across genotypes.

引用

页码：181 / 184

页数：4

共 50 条

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