Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein

被引：10
|
作者：
Hao, Meng [1 ,2 ,3 ]
Zhang, Guanying [3 ]
Zhang, Shengnan [4 ]
Chen, Zhengshan [5 ]
Chi, Xiangyang [3 ]
Dong, Yunzhu [3 ]
Fan, Pengfei [3 ]
Liu, Yujiao [3 ]
Chen, Yi [3 ]
Song, Xiaohong [3 ]
Liu, Shuling [3 ]
Yu, Changming [3 ]
Li, Jianmin [3 ]
Xia, Xianzhu [1 ,2 ,6 ]
机构：
[1] Chinese Acad Med Sci, Comparat Med Ctr, Peking Union Med Coll, Beijing 100021, Peoples R China
[2] Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing 100021, Peoples R China
[3] Beijing Inst Biotechnol, Beijing 100071, Peoples R China
[4] Northeast Forestry Univ, Coll Wildlife & Protected Areas, Harbin 150040, Peoples R China
[5] Tianjin Institue Envionmental & Operat Med, Key Lab Risk Assessment & Control Environm & Food, Tianjin 300050, Peoples R China
[6] Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130000, Peoples R China
来源：
VIRUSES-BASEL | 2020年 / 12卷 / 03期
关键词：
Rift Valley fever virus (RVFV); neutralizing antibody (NAb); Gn protein; critical residues; MONOCLONAL-ANTIBODIES; EPIDEMIC; VACCINE; IDENTIFICATION;
D O I：
10.3390/v12030259
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Rift Valley fever virus Gn and Gc protein (rHAdV4-GnGcopt). The two NAbs were both able to protect host cells from RVFV infection. The interactions between NAbs and Gn were then characterized to demonstrate that these two NAbs might preclude RVFV glycoprotein rearrangement, hindering the exposure of fusion loops in Gc to endosomal membranes after the virus invades the host cell. The target region for the two NAbs is located in the Gn domain III, implying that Gn is a desired target for developing vaccines and neutralizing antibodies against RVFV.
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页数：15
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