A useful assay for transketolase in asymmetric syntheses

被引：19
|
作者：
Mitra, RK [1 ]
Woodley, JM [1 ]
机构：
[1] UNIV LONDON UNIV COLL,DEPT CHEM & BIOCHEM ENGN,ADV CTR BIOCHEM ENGN,LONDON WC1E 7JE,ENGLAND
来源：
BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 03期
关键词：
D O I：
10.1007/BF00158940
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and rapid HPLC assay is reported for the measurement and kinetic study of transketolase. The assay consists of monitoring the rate of conversion of beta-hydroxypyruvate and glycolaldehyde to L-erythrulose. The assay is also useful in monitoring transketolase catalysed biotransformations for all reactions which utilise B-hydroxypyruvate as a method of driving the reaction to completion.
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页码：167 / 172
页数：6
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