Simultaneous determination of vitamins B1, B2, B6, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis

A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B-1, B-2, B-6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water (20:80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer (pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was -10 kV. The detection wavelength of vitamins B-1, B-2, B-6, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B-1, B-2, B-6, nicotinamide, and calcium pantothenate in the correlation coefficients (r) range of 0.9968-0.9998. The limits of detection (LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1% - 98.9% with relative standard deviations (RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B-1, B-2, B-6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.