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Omics-based approaches reveal phospholipids remodeling of Rhizopus oryzae responding to furfural stress for fumaric acid-production from xylose
被引:11
|作者:
Pan, Xinrong
Liu, Huanhuan
Liu, Jiao
Wang, Cheng
Wen, Jianping
[1
]
机构:
[1] Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China
基金:
中国国家自然科学基金;
关键词:
Furfural;
R;
oryzae;
Fumaric acid;
Metabolomics;
Lipidomics;
ENVIRONMENTAL-STRESSES;
MEMBRANE;
YEAST;
FERMENTATION;
OPTIMIZATION;
CEREVISIAE;
INHIBITORS;
TOLERANCE;
LIPIDS;
ROLES;
D O I:
10.1016/j.biortech.2016.09.101
中图分类号:
S2 [农业工程];
学科分类号:
0828 ;
摘要:
In order to relieve the toxicity of furfural on Rhizopus oryzae fermentation, the molecular mechanism of R. oryzae responding to furfural stress for fumaric acid-production was investigated by omics-based approaches. In metabolomics analysis, 29 metabolites including amino acid, sugars, polyols and fatty acids showed significant changes for maintaining the basic cell metabolism at the cost of lowering fumaric acid production. To further uncover the survival mechanism, lipidomics was carried out, revealing that phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and polyunsaturated acyl chains might be closely correlated with R. oryzae's adapting to furfural stress. Based on the above omics analysis, lecithin, inositol and soybean oil were exogenously supplemented separately with an optimized concentration in the presence of furfural, which increased fumaric acid titer from 5.78 g/L to 10.03 g/L, 10.05 g/L and 12.13 g/L (increased by 73.5%, 73.8% and 110%, respectively). These findings provide a methodological guidance for hemicellulose-fumaric acid development. (C) 2016 Elsevier Ltd. All rights reserved.
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页码:24 / 32
页数:9
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