Enzyme-catalyzed preparative peptide synthesis in frozen aqueous systems

被引：8

作者：

Haensler, M ^{[1
]}

Thust, S ^{[1
]}

Klossek, P ^{[1
]}

Ullmann, G ^{[1
]}

机构：

[1] Univ Leipzig, Inst Biochem, Fac Biosci Pharm & Psychol, D-04103 Leipzig, Germany

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1999年 / 6卷 / 1-2期

关键词：

protease; preparative peptide synthesis; frozen aqueous system;

D O I：

10.1016/S1381-1177(98)00135-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Chymotrypsin (alpha-CT; EC 3.4.21.1) and papain (EC 3.4.22.2) were used as catalysts in preparative peptide synthesis in frozen aqueous systems. A special apparatus was constructed in order to enable shock freezing of large reaction volumes. Several hundred milligram of peptides including non-natural components were synthesized representing yields of 43% to 95%. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 98

页数：4

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