Cγ-Aminopropylene peptide nucleic acid (amp-PNA): chiral cationic PNAs with superior PNA:DNA/RNA duplex stability and cellular uptake

被引：19

作者：

Kumar, Pramod ^{[1
]}

Jain, Deepak R. ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res, Chem Biol Unit, Pune 411008, Maharashtra, India

来源：

TETRAHEDRON | 2015年 / 71卷 / 21期

关键词：

Cationic PNA; Spacer chain; DNA/RNA hybridization; Cell penetration; HYBRIDIZATION PROPERTIES; ANALOGS; DNA; ANTISENSE; BACKBONE; DELIVERY; BINDING; DNA/RNA; GENE;

D O I：

10.1016/j.tet.2015.03.093

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Inherently cationic and chiral C-gamma-aminopropylene peptide nucleic acid (amp-PNA) have been synthesized with three carbon spacer chain from the backbone. The modified amp-PNAs have shown to stabilize (Delta T-m) the PNA:DNA duplexes better than that of corresponding PNA:RNA duplexes. However, the PNA:RNA duplexes have higher thermal stability (T-m) compared to PNA:DNA duplexes for both modified and unmodified PNAs. The live cell imaging of amp-PNAs demonstrated their effective penetration inside the MCF-7 cells and found to be accumulated in the vicinity of the nuclear membrane in the cytoplasm. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：3378 / 3384

页数：7

共 23 条

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