Effects of methyl bromide fumigation on community structure of denitrifying bacteria with nitrous oxide reductase gene (nosZ) in soil

被引:1
|
作者
Yan Pingmei [1 ,2 ]
Zhang Teng [1 ]
Li Yuan [1 ]
Yan Dongdong [1 ]
Mao Liangang [1 ]
Wan Qiuxia [1 ]
Guo Meixia [1 ]
Cao Aocheng [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China
[2] Taiyuan Normal Univ, Taiyuan 030012, Shanxi, Peoples R China
来源
基金
美国国家科学基金会;
关键词
Methyl bromide fumigation; denitrifying bacteria; nosZ gene; community structure; DIVERSITY; CHLOROPICRIN;
D O I
10.5897/AJMR11.1377
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Soil fumigation can inevitably affect the activity of non-target soil microorganisms, the effects of the methyl bromide fumigation on community structure and activity of denitrifying bacteria in soil were studied by nosZ-PCR-RFLP (restriction fragment length polymorphism) method. The results showed that Margalef index, Shannon-wiener index and Evenness index of methyl bromide fumigation soil and nosZ -denitrifying bacteria significantly different, the number of denitrifying bacteria in CK soil was 1.4 times higher than soil fumigated through nosZ-MPN-PCR (Most-Probable-Number-PCR), and denitrification activity of soil fumigated by methyl bromide was very significantly higher than the control soil using soil nitrate eradication.
引用
收藏
页码:2095 / 2100
页数:6
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