Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia

被引:97
|
作者
Zhu, L
Ang, S
Liu, WT
机构
[1] Natl Univ Singapore, Dept Civil Engn, Singapore 117576, Singapore
[2] Natl Univ Singapore, Dept Mech Engn, Singapore 117576, Singapore
[3] Univ Arkansas, Dept Elect Engn, Fayetteville, AR 72701 USA
[4] Univ Arkansas, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA
关键词
D O I
10.1128/AEM.70.1.597-598.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C parvum, and allowed dual-color detection for C. parvum and G. lamblia.
引用
收藏
页码:597 / 598
页数:2
相关论文
共 50 条
  • [1] Settling velocity of Cryptosporidium parvum and Giardia lamblia
    Dai, X
    Boll, J
    WATER RESEARCH, 2006, 40 (06) : 1321 - 1325
  • [2] Evaluation of attachment of Cryptosporidium parvum and Giardia lamblia to soil particles
    Dai, X
    Boll, J
    JOURNAL OF ENVIRONMENTAL QUALITY, 2003, 32 (01) : 296 - 304
  • [3] Comparison of Primers and Optimization of PCR Conditions for Detection of Cryptosporidium parvum and Giardia lamblia in Water
    Rochelle, P. A.
    De Leon, R.
    Stewart, M. H.
    Wolfe, R. L.
    Applied and Environmental Microbiology, 63 (01):
  • [4] Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water
    Rochelle, PA
    DeLeon, R
    Stewart, MH
    Wolfe, RL
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) : 106 - 114
  • [5] Combined FISH and mAb detection of Cryptosporidium parvum and Giardia lamblia carried by synanthropic flies
    Graczyk, TK
    Grimes, BH
    Knight, RL
    Da Silva, AJ
    Pieniazek, NJ
    Veal, DA
    PROCEEDINGS OF THE 10TH INTERNATIONAL CONGRESS OF PARASITOLOGY-ICOPA X: SYMPOSIA, WORKSHOPS AND CONTRIBUTED PAPERS, 2002, : 591 - 595
  • [6] Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum
    Elsafi, Salah H.
    Al-Maqati, Thekra N.
    Hussein, Mohi I.
    Adam, Ahmed A.
    Abu Hassan, Mohamed M.
    Al Zahrani, Eidan M.
    PARASITOLOGY RESEARCH, 2013, 112 (04) : 1641 - 1646
  • [7] Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum
    Salah H. Elsafi
    Thekra N. Al-Maqati
    Mohi I. Hussein
    Ahmed A. Adam
    Mohamed M. Abu Hassan
    Eidan M. Al Zahrani
    Parasitology Research, 2013, 112 : 1641 - 1646
  • [8] Detection of Giardia lamblia and Cryptosporidium parvum in water samples using multiplex sequencing technology
    Vinayagamoorthy, T
    Mulatz, K
    Wallis, P
    Hodkinson, R
    AMERICAN LABORATORY, 2004, 36 (10) : 46 - +
  • [9] Detection and genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by oligonucleotide microarray
    Wang, Z
    Vora, GJ
    Stenger, DA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) : 3262 - 3271
  • [10] Surface-Enhanced Resonance Raman Spectroscopy for the Rapid Detection of Cryptosporidium parvum and Giardia lamblia
    Rule, Krista L.
    Vikesland, Peter J.
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2009, 43 (04) : 1147 - 1152