Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract A role for antioxidants in skin health

被引：10

作者：

Rajagopalan, Pavithra ^{[1
]}

Jain, Ankit P. ^{[1
]}

Nanjappa, Vishalakshi ^{[1
]}

Patel, Krishna ^{[1
]}

Mangalaparthi, Kiran K. ^{[1
]}

Babu, Niraj ^{[1
]}

Cavusoglu, Nukhet ^{[2
]}

Roy, Nita ^{[3
]}

Soeur, Jeremie ^{[2
]}

Breton, Lionel ^{[2
]}

Pandey, Akhilesh ^{[4
,5
,6
,7
]}

Gowda, Harsha ^{[1
]}

Chatterjee, Aditi ^{[1
]}

Misra, Namita ^{[2
,3
]}

机构：

[1] Int Tech Pk, Inst Bioinformat, 7th Floor,Discoverer Bldg, Bangalore 560066, Karnataka, India

[2] LOreal Res & Innovat, F-93600 Aulnay Sous Bois, France

[3] LOreal India Pvt Ltd, Bearys Global Res Triangle, Bangalore 560067, Karnataka, India

[4] Johns Hopkins Univ, Sch Med, McKusick Nathans Inst Genet Med, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA

[6] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA

[7] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

来源：

JOURNAL OF DERMATOLOGICAL SCIENCE | 2019年 / 96卷 / 02期

关键词：

Pollution; Tocopherol; Orbitrap fusion; Quantitative proteomics; Skin keratinocytes; Electron transport chain; POLYCYCLIC AROMATIC-HYDROCARBONS; AIR-POLLUTION; EXHAUST PARTICLES; BARRIER FUNCTION; GENE-EXPRESSION; CELL MIGRATION; POLLUTANTS; COLLAGEN; METALLOPROTEINASES; CARCINOGENICITY;

D O I：

10.1016/j.jdermsci.2019.08.009

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background: Skin acts as a protective barrier against direct contact with pollutants but inhalation and systemic exposure have indirect effect on keratinocytes. Exposure to diesel exhaust has been linked to increased oxidative stress. Objective: To investigate global proteomic alterations in diesel particulate extract (DPE)/its vapor exposed skin keratinocytes. Methods: We employed Tandem Mass Tag (TMT)-based proteomics to study effect of DPE/DPE vapor on primary skin keratinocytes. Results: We observed an increased expression of oxidative stress response protein NRF2, upon chronic exposure of primary keratinocytes to DPE/its vapor which includes volatile components such as polycyclic aromatic hydrocarbons (PAHs). Mass spectrometry-based quantitative proteomics led to identification 4490 proteins of which 201 and 374 proteins were significantly dysregulated (>= 1.5 fold, p <= 0.05) in each condition, respectively. Proteins involved in cellular processes such as cornification (cornifin A), wound healing (antileukoproteinase) and differentiation (suprabasin) were significantly downregulated in primary keratinocytes exposed to DPE/DPE vapor. These results were corroborated in 3D skin models chronically exposed to DPE/DPE vapor. Bioinformatics analyses indicate that DPE and its vapor affect distinct molecular processes in skin keratinocytes. Components of mitochondrial oxidative phosphorylation machinery were seen to be exclusively overexpressed upon chronic DPE vapor exposure. In addition, treatment with an antioxidant like vitamin E partially restores expression of proteins altered upon exposure to DPE/DPE vapor. Conclusions: Our study highlights distinct adverse effects of chronic exposure to DPE/DPE vapor on skin keratinocytes and the potential role of vitamin E in alleviating adverse effects of environmental pollution. (C) 2019 The Author(s). Published by Elsevier B.V. on behalf of Japanese Society for Investigative Dermatology.y

引用

页码：114 / 124

页数：11

共 2 条

[1] Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract-A role for antioxidants in skin health
Rajagopalan, Pavithra
Jain, Ankit P.
Nanjappa, Vishalakshi
Patel, Krishna
Mangalaparthi, Kiran K.
Babu, Niraj
Cavusoglu, Nukhet
Roy, Nita
Soeur, Jeremie
Breton, Lionel
Pandey, Akhilesh
Gowda, Harsha
Chatterjee, Aditi
Misra, Namita
JOURNAL OF DERMATOLOGICAL SCIENCE, 2018, 91 (03) : 239 - 249
[2] Ceramide-1-phosphate accelerates premature skin aging in skin fibroblasts exposed to diesel particulate extract
Shin, K.
Kim, S.
Choi, Y.
Back, S.
Lee, H.
Kim, B.
Kim, J.
Uchida, Y.
Park, K.
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2022, 142 (08) : S122 - S122

← 1 →