Pulsed-field gel electrophoresis in differentiation of Erysipelothrix species strains

被引：14
|
作者：
Okatani, AT [1 ]
Uto, T [1 ]
Taniguchi, T [1 ]
Horisaka, T [1 ]
Horikita, T [1 ]
Kaneko, K [1 ]
Hayashidani, H [1 ]
机构：
[1] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Tokyo 1838509, Japan
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 11期
关键词：
D O I：
10.1128/JCM.39.11.4032-4036.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We report here the first analysis of Erysipelothrix spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of Erysipelothrix spp. were analyzed. SmaI, AscI, and NotI were tested for the ability to cleave the DNA extracted from those strains, and among them, SmaI was the most reliable enzyme. Sixty-three distinct PFGE patterns were produced, and no DNA degradation was observed, allowing the identification of all of the strains. Based on these results and on those of a previous analysis using randomly amplified polymorphic DNA and ribotyping, PFGE with SmaI might be considered to be more sensitive than those methods and to be the best method for epidemiological studies of strains of this genus.
引用
收藏
页码：4032 / 4036
页数：5
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