Modified Rat Hepatocellular Carcinoma Models Overexpressing Vascular Endothelial Growth Factor

被引:10
|
作者
Choi, Jin Woo [1 ]
Cho, Hye Rim [1 ]
Lee, Kyoungbun [2 ]
Jung, Jae Kyung [1 ]
Kim, Hyo-Cheol [1 ]
机构
[1] Seoul Natl Univ, Seoul Natl Univ Hosp, Coll Med, Dept Radiol, 101 Daehak Ro, Seoul 03080, South Korea
[2] Seoul Natl Univ, Seoul Natl Univ Hosp, Coll Med, Dept Pathol, 101 Daehak Ro, Seoul 03080, South Korea
基金
新加坡国家研究基金会;
关键词
TRANSARTERIAL CHEMOEMBOLIZATION; INTRAARTERIAL DELIVERY; COMPUTED-TOMOGRAPHY; HEPATOMA; EMBOLIZATION; PERFUSION; SURVIVAL; CELLS; MRI;
D O I
10.1016/j.jvir.2018.07.005
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose: To compare tumor vascularity in 4 types of rat hepatocellular carcinoma (HCC) models: NISI, vascular endothelial growth factor (VEGF)-transfected NISI (VEGF-N1S1), McA-RH7777, and VEGF-transfected McA-RH7777 (VEGF-McA-RH777) tumors. Materials and Methods: The N1S1 and McA-RH7777 cell lines were transfected with expression vectors containing cDNA for rat VEGF. Eighty-eight male Sprague-Dawley rats (weight range, 400-450 g) were randomly divided into 4 groups (ie, 22 rats per model), and 4 types of tumor models were created by using the N1S1, VEGF-N1S1, McA-RH7777, and VEGF-McA-RH777 cell lines. Tumor vascularity was evaluated by perfusion computed tomography (CT), enzyme-linked immunosorbent assay of VEGF, CD34 staining, angiography, and Lipiodol transarterial embolization. Intergroup discrepancies were evaluated by Kruskal-Wallis test. Results: Arterial perfusion (P < .001), portal perfusion (P = .015), total perfusion (P < .001), tumor VEGF level (P = .002), and microvessel density (MVD; P = .007) were significantly different among groups. VEGF-McA-RH7777 tumors showed the greatest arterial perfusion (46.7 mL/min/100 mL +/--15.5), total perfusion (60.7 mL/min/100 mL +/- 21.8), tumor VEGF level (3,376.7 pg/mL +/- 145.8), and MVD (34.5%o +/- 7.5). Whereas most tumors in the NISI, VEGF-N1S1, and McA-RH7777 groups showed hypovascular staining on angiography and minimal Lipiodol uptake after embolization, 5 of 6 VEGF-McA-RH7777 tumors (83.3%) presented hypervascular tumor staining and moderate to compact Lipiodol uptake. Conclusions: McA-RH7777 tumors were more hypervascular than N1S1 tumors, and tumor vascularity was enhanced further by VEGF transfection. Therefore, the VEGF-McA-RH7777 tumor is recommended to mimic hypervascular human HCC in rats.
引用
收藏
页码:1604 / 1612
页数:9
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