Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells

被引：0
|
作者：
Koj, A [1 ]
Jura, J [1 ]
机构：
[1] Jagiellonian Univ, Fac Biotechnol, Dept Cellular Biochem, PL-30387 Krakow, Poland
来源：
ACTA BIOCHIMICA POLONICA | 2003年 / 50卷 / 03期
关键词：
acute phase response; inflammatory reaction; hepatoma cells; gene expression; transcriptome; proteome; differential display; microarray; ACUTE-PHASE RESPONSE; BLOOD MONONUCLEAR-CELLS; TUMOR-NECROSIS-FACTOR; SERIAL ANALYSIS; MESSENGER-RNA; EXPRESSED GENES; IDENTIFICATION; HYBRIDIZATION; INHIBITION; MICROARRAY;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-l-beta (IL-1beta) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1beta to evaluate the transcriptome induced by this cytokine during 24 h of treatment. By using differential display analysis we found IL-1beta-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or involved in posttranscription/posttranslation modifications, proteases, proteins involved in cellular metabolism, activity modulators, proteins of the cell cycle machinery and also some new proteins so far functionally not classified.
引用
收藏
页码：573 / 582
页数：10
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