Nonionic surfactants induced cloud point extraction of Polyhydroxyalkanoate from Cupriavidus necator

被引：8
|
作者：
Murugesan, Sivananth [1 ]
Iyyasamy, Regupathi [1 ]
机构：
[1] Natl Inst Technol Karnataka, Dept Chem Engn, Mangalore 575025, India
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2017年 / 52卷 / 12期
关键词：
Cloud point extraction; Cupriavidus necator; nonionic surfactant; polyhydroxyalkanoate (PHA); HYDROPHILE-LIPOPHILE BALANCE; MEDIATED PHASE SEPARATIONS; AQUEOUS MICELLAR-SOLUTIONS; MEMBRANE-PROTEINS; SYSTEMS; DISRUPTION; RECOVERY; BEHAVIOR; BIOMASS; SALT;
D O I：
10.1080/01496395.2017.1307227
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Polyhydroxyalkanoate synthesized by Cupriavidus necator DSM 428 was purified from the crude fermentation broth as such by performing nonionic surfactants (Triton X100, Triton X114 & Tergitol 6) induced cloud point extraction. Polyhydroxyalkanoate was extracted into the micelle-rich bottom phase (coacervate phase), while most of the cellular impurities partitioned into the aqueous phase. Cloud point temperatures and the extraction efficiency of different cloud point systems were studied at different pH value and in the presence of additives. Maximum extraction of biopolymer was achieved (recovery of 84.4%) with a purity of 92.49% at 3 pH with the addition of 0.1M ammonium chloride in the mixed surfactant system at a reduced cloud point temperature of 33 degrees C.
引用
收藏
页码：1929 / 1937
页数：9
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