Photobioreactor strategies for improving the CO2 fixation efficiency of indigenous Scenedesmus obliquus CNW-N: Statistical optimization of CO2 feeding, illumination, and operation mode

被引:47
|
作者
Ho, Shih-Hsin [1 ]
Lu, Wei-Bin [2 ]
Chang, Jo-Shu [1 ,3 ,4 ,5 ]
机构
[1] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan
[2] Chung Hwa Univ Med Technol, Dept Cosmet Sci, Tainan, Taiwan
[3] Natl Cheng Kung Univ, Ctr Biosci & Biotechnol, Tainan 70101, Taiwan
[4] Natl Cheng Kung Univ, Res Ctr Energy Technol, Tainan 70101, Taiwan
[5] Natl Cheng Kung Univ, Strategy Ctr, Tainan 70101, Taiwan
关键词
Microalgae; Scenedesmus obliquus; CO2; fixation; Response surface methodology; Semi-batch culture; CARBON-DIOXIDE; SPIRULINA-PLATENSIS; MICROALGAE; GROWTH; LIGHT; CULTIVATION; BIOFIXATION; MITIGATION; VULGARIS; BIOMASS;
D O I
10.1016/j.biortech.2011.11.091
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Statistical experimental design and bioreactor strategies were applied to enhance CO2 fixation ability of microalga Scenedesmus obliquus CNW-N. Four operating parameters strongly influencing microalgal CO2 fixation efficiency (namely, CO2 concentration, CO2 flow rate, magnesium concentration, and light intensity) were optimized with response surface methodology. The optimal range of parameters achieving the best overall performance of specific growth rate and CO2 fixation rate was determined with overlay counter plot techniques. Optimal ranges of CO2 concentration, CO2 flow rate, magnesium concentration and light intensity were 2.0-2.5%, 0.3-0.5 vvm, 1.7-2.7 mM and 180-250 mu mol m(-2) s(-1), respectively, achieving a specific growth rate of >1.22 d(-1) and CO2 fixation rate of >800 mg L-1 d(-1). Semi-batch operations further enhanced the biomass productivity, photosynthesis efficiency, and CO2 fixation rate to 1030 mg L-1 d(-1), 10.5%, and 1782 mg L-1 d(-1), respectively. This performance is better than the results reported by most related studies. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:106 / 113
页数:8
相关论文
共 42 条
  • [1] Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation
    Ho, Shih-Hsin
    Kondo, Akihiko
    Hasunuma, Tomohisa
    Chang, Jo-Shu
    BIORESOURCE TECHNOLOGY, 2013, 143 : 163 - 171
  • [2] Scenedesmus obliquus CNW-N as a potential candidate for CO2 mitigation and biodiesel production
    Ho, Shih-Hsin
    Chen, Wen-Ming
    Chang, Jo-Shu
    BIORESOURCE TECHNOLOGY, 2010, 101 (22) : 8725 - 8730
  • [3] Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N
    Ho, Shih-Hsin
    Chen, Chun-Yen
    Chang, Jo-Shu
    BIORESOURCE TECHNOLOGY, 2012, 113 : 244 - 252
  • [4] Bioprocess development on microalgae-based CO2 fixation and bioethanol production using Scenedesmus obliquus CNW-N
    Ho, Shih-Hsin
    Li, Po-Jen
    Liu, Chen-Chun
    Chang, Jo-Shu
    BIORESOURCE TECHNOLOGY, 2013, 145 : 142 - 149
  • [5] Biosorption of cadmium by CO2-fixing microalga Scenedesmus obliquus CNW-N
    Chen, Chun-Yen
    Chang, Hao-Wei
    Kao, Pei-Chun
    Pan, Jian-Liang
    Chang, Jo-Shu
    BIORESOURCE TECHNOLOGY, 2012, 105 : 74 - 80
  • [6] Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes
    Ho, Shih-Hsin
    Chen, Yi-Di
    Chang, Ching-Yu
    Lai, Yen-Ying
    Chen, Chun-Yen
    Kondo, Akihiko
    Ren, Nan-Qi
    Chang, Jo-Shu
    BIOTECHNOLOGY FOR BIOFUELS, 2017, 10
  • [7] Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes
    Shih-Hsin Ho
    Yi-Di Chen
    Ching-Yu Chang
    Yen-Ying Lai
    Chun-Yen Chen
    Akihiko Kondo
    Nan-Qi Ren
    Jo-Shu Chang
    Biotechnology for Biofuels, 10
  • [8] INFLUENCE OF TRIS ON CO2 FIXATION IN MAIZE, SCENEDESMUS OBLIQUUS AND ISOLATED CHLOROPLASTS
    POPOVA, LP
    VAKLINOV.SG
    DOKLADI NA BOLGARSKATA AKADEMIYA NA NAUKITE, 1972, 25 (03): : 393 - &
  • [9] Operational strategies for maximizing CO2 utilization efficiency by the novel microalga Scenedesmus obliquus SA1 cultivated in lab scale photobioreactor
    Basu, Samarpita
    Roy, Abhijit Sarma
    Ghoshal, Aloke K.
    Mohanty, Kaustubha
    ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2015, 12 : 249 - 257
  • [10] Enhancing Scenedesmus obliquus biofilm growth and CO2 fixation in a gas-permeable membrane photobioreactor integrated with additional rough surface
    Guo, Chenglong
    Duan, Danru
    Sun, Yahui
    Han, Yuying
    Zhao, Sha
    ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2019, 43