Identification and selection of a new Saccharomyces cerevisiae strain isolated from Brazilian ethanol fermentation process for application in beer production

被引:9
|
作者
Mandujano, Gustavo P. Lorca [1 ,3 ]
Alves, Henrique C. [1 ]
Prado, Cleiton D. [1 ]
Martins, Jeferson G. O. [1 ,3 ]
Novaes, Hosana R. [1 ]
Maia de Oliveira da Silva, Joao Pedro [1 ]
Teixeira, Gleidson Silva [4 ]
Ohara, Andre [4 ]
Alves, Mateus H. R. [1 ]
Pedrino, Isadora C. [1 ]
Malavazi, Iran [1 ]
de Sousa, Cristina Paiva [2 ,3 ]
da Cunha, Anderson F. [1 ,3 ]
机构
[1] Univ Fed Sao Carlos UFSCar, Genet & Evolut Dept, BR-13565905 Sao Carlos, SP, Brazil
[2] Univ Fed Sao Carlos UFSCar, Morphol & Pathol Dept, BR-13565905 Sao Carlos, SP, Brazil
[3] Univ Fed Sao Carlos UFSCar, Biotechnol Grad Program, BR-13565905 Sao Carlos, SP, Brazil
[4] ITAL Tecnolat, Custom Made Yeasts, Bioinfood, BR-13070178 Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
LBGA-287; strain; Beer production; New yeast strain; Brazilian ethanol fermentation; Saccharomyces; PCR; GLYCEROL; DEHYDROGENASE; PASTORIANUS; BIOETHANOL; EXPRESSION; INSIGHTS; YEASTS; SUGAR; GPD1;
D O I
10.1016/j.fm.2021.103958
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The fermented beverage industry is always pursuing alternatives to make products that delight consumers with special or unique characteristics. The identification and improvement of new yeast strains emerge as an opportunity; however, wild strains usually have a limitation in maltose fermentation and/or off-flavors production. Here we report the production of a Blond-style ale beer using a bioethanol isolated strain (LBGA-287) with flavor complexity approved in sensorial panels. LBGA-287 also showed an increase in maltose consumption, growth and fermentation rates when compared to the commercial yeast. Using qPCR analysis, genes related to the (i) efficiency of fermentation (ii) production of aromas/off-flavors, and (iii) metabolization of carbohydrates were found as differentially expressed in the isolated strains when compared to industrial yeast. This suggests that LBGA-287 could have an important impact on beer production, improving brewing efficiency, quality and diversity of this beverage, and most importantly satisfying the final consumer.
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页数:10
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