Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

被引：9

作者：

Laomeephol, Chavee ^{[1
]}

Vasuratna, Apichai ^{[2
]}

Ratanavaraporn, Juthamas ^{[1
,3
,4
]}

Kanokpanont, Sorada ^{[1
,3
,5
]}

Luckanagul, Jittima Amie ^{[1
,6
]}

Humenik, Martin ^{[7
]}

Scheibel, Thomas ^{[7
]}

Damrongsakkul, Siriporn ^{[1
,3
,5
]}

机构：

[1] Chulalongkorn Univ, Fac Engn, Biomat Engn Med & Hlth Res Unit, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Fac Med, Dept Obstet & Gynecol, Bangkok 10330, Thailand

[3] Chulalongkorn Univ, Fac Engn, Biomed Engn Res Ctr, Bangkok 10330, Thailand

[4] Chulalongkorn Univ, Fac Engn, Biomed Engn Program, Bangkok 10330, Thailand

[5] Chulalongkorn Univ, Fac Engn, Dept Chem Engn, Bangkok 10330, Thailand

[6] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Pharmaceut & Ind Pharm, Bangkok 10330, Thailand

[7] Univ Bayreuth, Fac Engn Sci, Dept Biomat, Prof Rudiger Bormann Str 1, D-95447 Bayreuth, Germany

来源：

POLYMERS | 2021年 / 13卷 / 23期

关键词：

silk fibroin; spider silk; hydrogel; self-assembly; cell culture; PROTEIN; GELATION; PROLIFERATION; MECHANISM; SURFACE;

D O I：

10.3390/polym13234182

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.

引用

页数：14

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