Partial characterization of a lipase from gypsy moth (Lymantria dispar L.) larval midgut

被引：17

作者：

Mrdakovic, Marija ^{[1
]}

Lazarevic, Jelica ^{[1
]}

Peric-Mataruga, Vesna ^{[1
]}

Ilijin, Larisa ^{[1
]}

Vlahovic, Milena ^{[1
]}

机构：

[1] Inst Biol Res, Belgrade 11000, Serbia

来源：

FOLIA BIOLOGICA-KRAKOW | 2008年 / 56卷 / 1-2期

关键词：

Lymantria dispar L; lipase; p-nitrophenyl esters; optimal conditions; inhibition;

D O I：

10.3409/fb56_1-2.103-110

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lipase activity of the gypsy moth (Lymantria dispar L.) was studied by the spectrophotometric method using crude homogenate of fifth-instar larval midgut tissues as the enzyme source and p-nitrophenyl caprylate (pNPC) as substrate. A Km value of 0.310mM and a Vmax value of 1.479U/mg Prot. were obtained for this substrate. Among various p-nitrophenyl esters tested, maximum activity was obtained for p-nitrophenyl caprylate and p-nitrophenyl caprate. The enzyme was most active at alkaline pH, with maximum at pH 8.2. Decreased activity was detected after preincubation in buffers of PH below 7.0 and above 8.2. The enzyme was unstable at room temperature. The enzyme was Ca2+ independent. Its activity was inhibited by PMSF, Fe2+, Ag+ and Ph2+, while Fe3+ inhibited enzyme activity by about 40%.

引用

页码：103 / 110

页数：8

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