Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene

被引：21
|
作者：
Park, HS
Lee, JH
Jeong, EJ
Kim, JE
Hong, SJ
Park, TK
Kim, TY
Jang, WJ
Park, KH
Kim, BJ
Kook, YH
Lee, SH
机构：
[1] Konkuk Univ, Dept Microbiol, Coll Med, Chungju 380701, Chungchongbuk D, South Korea
[2] Konkuk Univ, Dept Pediat, Coll Med, Chungju 380701, Chungchongbuk D, South Korea
[3] Konkuk Univ, Dept Biotechnol, Chungju 380701, Chungchongbuk D, South Korea
[4] Minist Agr & Forestry, Div Anim Hlth, Kwacheon 427719, South Korea
[5] Seoul Natl Univ, Coll Med, Dept Microbiol, Seoul 110799, South Korea
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2005年 / 49卷 / 06期
关键词：
groEL gene; Rickettsia; duplex PCR;
D O I：
10.1111/j.1348-0421.2005.tb03760.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
引用
收藏
页码：545 / 549
页数：5
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