Modified Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction Using Methyl Benzoate for the Preconcentration of Fungicides in Fruit Juice Samples and Determination by High-Performance Liquid Chromatography

Methyl benzoate-based vortex-assisted surfactant-enhanced emulsification microextraction (VASEME) has been developed and applied for the preconcentration of fungicides (i.e., carbendazim, thiabendazole and fluberidazole) and their subsequent determination by high performance liquid chromatography. Methyl benzoate (as extraction solvent) and sodium dodecyl sulfate (as emulsifier) were used in VASEME. Parameters affecting the extraction performance were investigated and optimized. Under the optimum conditions, linearity was obtained in the range of 0.1-200 mu g/L with the coefficient of determination exceeding 0.998. Limits of detection and quantitation were 0.01-0.5 and 0.1-3 mu g/L, respectively. Recoveries of the spiked analytes (10-100 mu g/L) from fruit juice samples were between 72 and 116% with the relative standard deviation below 10%. The proposed method is simple, rapid, consuming less solvent, sensitive and reliable for the determination of fungicide residues in fruit juice samples.