Chiral separation of trimebutine maleate by using affinity capillary electrophoresis with human serum albumin

Affinity capillary electrophoresis has been used for the enantiomeric separation of racemic trimebutine maleate with bare fused capillary using human serum albumin(HSA) as chiral selector. The running buffer was 50 mmol/L NaH2 PO4 (pH 5.55) and the separation buffer was 500 mu mol/l, HSA in the running buffer. The partial filling technique was employed in order to eliminate detection disturbance caused by high concentration of HSA. The filling time of the separation buffer was 160s, applied voltage was 9.0 kV, ultraviolet detector was set at 214 Mn. A chiral separation with a resolution of 1.2 was achieved in above conditions. The factors which influenced the enantiomeric resolution such as type of chiral selector, source and concentration of HSA, pH of running buffer, partial filling time of separation buffer and organic modifiers were investigated.