Starvation effects on nitrogen and carbon stable isotopes of animals: an insight from meta-analysis of fasting experiments

Nitrogen and carbon stable isotopic compositions (delta N-15 and delta C-13) of consumers have been used for physiological and food web studies. Previous studies have shown delta N-15 and delta C-13 values are affected by several biological and environmental factors during starvation, but the generality of the effect of starvation on delta N-15 and delta C-13 values has not yet been tested. Here, we performed a meta-analysis to evaluate the effects of starvation on delta N-15 and delta C-13 values of consumers, and the underlying factors that may explain the observed variation. The delta N-15 and delta C-13 values were calculated as the differences between the final delta N-15 and delta C-13 values of consumers (post-starvation) and the pre-starvation values on each experiment. Our meta-analysis showed a large variation in the delta N-15 and delta C-13 values of consumers ( delta N-15 range: -0.82 to 4.30 parts per thousand; mean: 0.47% and delta C-13 range: -1.92 to 2.62 parts per thousand; mean: 0.01 parts per thousand). The delta N-15 values of most consumers increased along the length of the starvation period and were influenced by nitrogen excretion and thermoregulation types, probably because differences in nitrogen metabolism and thermoregulation affect nitrogen processing and excretion rates. None of our predictor variables accounted for the variation in delta C-13 values, which showed both increases and decreases due to fasting. Our findings suggest that starvation results in changes in consumer delta N-15 values which are mainly explained by the length of the fasting period and by nitrogen and energy metabolism, but the underlying mechanisms of the starvation effects on delta C-13 values seem to be more complex than previously thought.