Determination of Six Metal Elements in Animal Samples by Flame Atomic Absorption Spectrometry with Wet Digestion

The contents of 6 metal elements (Ca, Mg, K, Na, Cu and Fe) were determined in serum, urine, feces and different tissue and organs (heart, kidney, stomach, liver, spleen, intestine and lung) of Wistar rats and mice by flame atomic absorption spectrometry (FAAS) with wet digestion. The samples were digested by the mixture of HNO3 and HClO4 (4 : 1, V/V) at 120 degrees C, the correlation coefficient for the standard curves was 0. 999 4 similar to 0. 999 8, the relative standard deviation (RSD) was from 0. 33% to 4. 52%, and the recovery for the samples was from 97. 7% to 104. 2%, meeting the demand of elements content determination in biological samples. The assay method for the determination of the 6 metal elements in animal samples established in this study has the advantages of easy operation, high sensitivity, less sample requiring and accurate results, and the method can be widely used in the determination of various metal elements in biological samples.