Identification, isolation, and characterization of a species-specific 30-kDa antigen of Oesophagostomum dentatum

被引：8
|
作者：
Joachim, A
Ruttkowski, B
Christensen, CM
Daugschies, A
机构：
[1] Hannover Sch Vet Med, Inst Parasitol, D-30559 Hannover, Germany
[2] Royal Vet & Agr Univ, Danish Ctr Expt Parasitol, DK-1870 Frederiksberg C, Denmark
来源：
PARASITOLOGY RESEARCH | 1999年 / 85卷 / 04期
关键词：
D O I：
10.1007/s004360050553
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
In the search for a serology tool for the diagnosis of nonpatent as well as patent infections with Oesophagostomum dentatum in pigs a water-soluble, unglycosilated antigen of about 30 kDa specific for the third-stage larvae of the parasite was purified by ion-exchange chromatography. In Western blots, the antigen was first detected by antibodies at day 7 postinfection. Cross-reactivity with O. quadrispinulatum, Ascaris suum, or Trichuris suis was not detected. It is suggested that this protein is a suitable tool for the species-specific serodiagnosis of O. dentatum infection in pigs.
引用
收藏
页码：307 / 311
页数：5
相关论文
共 50 条
  • [1] Identification, isolation, and characterization of a species-specific 30-kDa antigen of Oesophagostomum dentatum
    A. Joachim
    B. Ruttkowski
    C. M. Christensen
    A. Daugschies
    Parasitology Research, 1999, 85 : 307 - 311
  • [2] CHARACTERIZATION OF THE 30-KDA CALCIMEDIN
    SMITH, VL
    DEDMAN, JR
    FASEB JOURNAL, 1988, 2 (04): : A554 - A554
  • [3] ISOLATION OF SPECIES-SPECIFIC ANTIGEN OF MYCOBACTERIA
    BOBROVNIK, SA
    LYASHCHENKO, KP
    GORBENKO, EV
    KOMISSARENKO, SV
    DOPOVIDI AKADEMII NAUK UKRAINSKOI RSR SERIYA B-GEOLOGICHNI KHIMICHNI TA BIOLOGICHNI NAUKI, 1990, (05): : 59 - 60
  • [4] ISOLATION AND PARTIAL CHARACTERIZATION OF A GENUS COMMON ANTIGEN AND SPECIES-SPECIFIC ANTIGEN OF CAPNOCYTOPHAGA
    MURAYAMA, Y
    MASHIMO, PA
    TABAK, LA
    LEVINE, MJ
    ELLISON, SA
    JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1982, 35 (04): : 153 - 170
  • [5] ISOLATION, CHARACTERIZATION, AND MOLECULAR-CLONING OF A SPECIFIC MYCOBACTERIUM-TUBERCULOSIS ANTIGEN GENE - IDENTIFICATION OF A SPECIES-SPECIFIC SEQUENCE
    PARRA, CA
    LONDONO, LP
    DELPORTILLO, P
    PATARROYO, ME
    INFECTION AND IMMUNITY, 1991, 59 (10) : 3411 - 3417
  • [6] ISOLATION AND CHARACTERIZATION OF A 30-KDA MEMBRANE GLYCOPROTEIN FROM HUMAN STRATUM-CORNEUM
    CHEN, SJ
    RAJARAMAN, S
    MILLER, J
    KALMAZ, GD
    BRYSK, MM
    BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 881 (03) : 375 - 382
  • [7] Isolation, characterization, molecular cloning and amplification of a species-specific M-leprae antigen
    Soto, CY
    Moreno, PA
    Valencia, JT
    Bernal, MM
    Guzman, F
    Patarroyo, ME
    Murillo, LA
    INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES, 1999, 67 (04) : 392 - 402
  • [8] DETECTION OF A PUTATIVE 30-KDA LIGAND OF THE CLUSTER-2 ANTIGEN
    HELFRICH, W
    VANGEEL, M
    THE, TH
    DELEIJ, L
    INTERNATIONAL JOURNAL OF CANCER, 1994, : 70 - 75
  • [9] DETECTION AND ISOLATION OF A 30-KDA ABNORMAL PROTEIN IN AVIAN DYSTROPHIC MUSCLE
    KOBAYASHI, R
    TOYOSHIMA, I
    MASAMUNE, O
    TASHIMA, Y
    JOURNAL OF BIOCHEMISTRY, 1990, 107 (01): : 51 - 55
  • [10] A 120KDA-PROTEIN AS A SPECIES-SPECIFIC ANTIGEN FOR CAMPYLOBACTER-PYLORI
    APEL, I
    JACOBS, E
    KIST, M
    ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 1988, 268 (04): : 544 - 544
12345