Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo)

被引:4
|
作者
Vergara, Amado Manuel Canales [1 ]
Landi, Vincenzo [2 ]
Bermejo, Juan Vicente Delgado [1 ]
Martinez, Amparo Martinez [1 ,2 ]
Acosta, Patricia Carvantes [3 ]
Barros, Agueda Pons [4 ]
Bigi, Daniele [5 ]
Sponenberg, Phillip [6 ]
Helal, Mostafa [7 ]
Banabazi, Mohammad Hossein [8 ]
Vallejo, Maria Esperanza Camacho [9 ]
机构
[1] Univ Cordoba, Fac Vet Sci, Dept Genet, Cordoba, Spain
[2] Anim Breeding Consulting SL, Parque Cient Tecnol Cordoba, Cordoba, Spain
[3] Univ Veracruzana, Fac Med Vet & Zootecnia, Xalapa, Veracruz, Mexico
[4] Serv Millora Agr SEMILLA, Palma de Mallorca, Spain
[5] Univ Bologna, Dept Agr & Food Sci, Bologna, Italy
[6] Virginia Maryland Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA USA
[7] Cairo Univ, Dept Anim Prod, Giza, Egypt
[8] AREEO, Anim Sci Res Inst IRAN ASRI, Dept Biotechnol, Karaj, Iran
[9] Inst Invest & Formac Agr & Pesquera IFAPA, Cordoba, Spain
关键词
Meleagris gallopavo; microsatellites; biodiversity; power of exclusion; BREEDS; MARKERS; LOCI;
D O I
10.1080/1828051X.2020.1745695
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Domestic turkey production generally utilises only a few genetically improved lines, and local breeds are severely endangered as a result. Furthermore, the genetic resources of domestic turkeys have not been properly investigated, which could, ultimately, lead to the extinction of local breeds and negatively affect their corresponding genetic diversity and environmental adaptation. Although, several microsatellite markers have been designed for mapping and quantitative trait locus analysis, there is no standard panel of markers for genetic characterisation or genetic diversity assessment. Accordingly, the present study aimed to develop a set of polymorphic microsatellite markers that could be used for international turkey population studies. Thirty-nine microsatellites were selected based on polymorphism, DNA sequence and chromosome position, as well as on amplification efficiency, success rate and the absence of nonspecific amplification. The markers were screened using 105 DNA samples from local turkey breeds from Mexico, the United States, Italy, Brazil, Egypt and Spain. A total of 401 alleles were identified, with a mean number of alleles per marker of 10.28 +/- 4.25. All microsatellites were polymorphic, with at least four alleles and no more than 19 alleles. Furthermore, allelic richness ranged from 3.810 to 17.985, mean heterozygosity ranged from 0.452 +/- 0.229 to 0.667 +/- 0.265, polymorphic information content values ranged from 0.213 (MNT264) to 0.850 (RHT0024) and the mean Fis value was 0.322. Overall, the panel was highly polymorphic and exhibited moderate Hardy-Weinberg disequilibrium, thereby indicating its value as a tool for biodiversity and population structure studies that could play an important role in promoting the conservation of local turkey breeds.
引用
收藏
页码:392 / 398
页数:7
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