The Application Value of ApoE Gene Polymorphism in Alzheimer's Disease

To explore the association of apolipoprotein E polymorphism with Alzheimer's disease (AD), so as to provide possible research value for potential targeted therapy. 120 AD patients and 50 healthy volunteers were enrolled to extract fasting blood samples. ApoE gene polymorphism and blood lipids were tested in blood. ApoE gene and genotype frequency between AD group and control group were compared by PCR and sequencing methods. MMSE, CDR, and BPSD were used to determine the intelligence. ApoE genotype was detected by DNA microarray. epsilon 4 carrier accounted for 45% in AD group, which was significantly elevated compared with control group (12%) (P < 0.05). TG, TC, and LDL-C levels were increased, while HDL-C was reduced in epsilon 4 allele carriers (all P < 0.05). The MMSE scores of ApoE epsilon 4 genotype carriers in AD group were markedly lower than those of non-ApoE epsilon 4 genotype carriers (P < 0.05) and control (P < 0.01). The proportion of dementia in ApoE epsilon 4 genotype carriers from AD group was apparently higher than the epsilon 4 gene non-carriers (P < 0.05). The ApoE epsilon 4 gene is an AD risk factor. The changes of genotype and frequency of ApoE epsilon 4 gene are the main factors leading to abnormal lipid metabolism in AD patients, suggesting that ApoE epsilon 4 gene detection might be helpful for the early diagnosis and treatment of AD.